Determination of plasma oxalate with oxalate oxidase
نویسندگان
چکیده
منابع مشابه
Determination of urinary oxalate with commercially available oxalate oxidase.
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wid...
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We use oxalate oxidase from barley seedlings for the colorimetric determination of oxalate in plasma. The oxalate is converted to hydrogen peroxide, which, in the presence of peroxidase, is detected by a Trinder-like chromogenic system. Optimization of the assay, including deproteinization and elimination of interferences from reducing substrates, is described. Ascorbate additions (200 mumol/L)...
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متن کاملA novel urinary oxalate determination method via a catalase model compound with oxalate oxidase†
This work describes a novel method of urinary oxalate determination based on a catalase model compound MnL(H2O)2(ClO4)2 (L 1⁄4 bis(2-pyridylmethyl)amino)propionic acid). Urinary oxalate is first decomposed by the oxalate oxidase into carbon dioxide and hydrogen peroxide. The later is then disproportionated into water and oxygen by the catalase model compound forming a color compound, which can ...
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ژورنال
عنوان ژورنال: Clinica Chimica Acta
سال: 1984
ISSN: 0009-8981
DOI: 10.1016/0009-8981(84)90311-5